Journal: Journal of Undergraduate Neuroscience Education

Article Title: Dissecting the Molecular and Neural Circuit Bases of Behavior as an Introduction to Discovery-Driven Research; A Report on a Course-Based Undergraduate Research Experience

doi:

Figure Lengend Snippet:

Article Snippet: RESOURCE SOURCE IDENTIFIER Core Drosophila strains Genetic background control: w 1118 Bloomington Drosophila Stock Center 3605 Cass III md neuron driver: GAL4 19 – 12 36369 UAS-TNT: w[ * ]; P{w[+mC]=UAS-TeTxLC.tnt}E2 28837 UAS-ChETA: w * ; wgSp-1/CyO, P{Wee-P.ph0}BaccWee-P20; P{20XUAS-CHETA.YFP}attP2/TM6C, Sb1 Tb1 36495 Additional GAL4 and UAS-RNAi strains - Materials, Reagents, and Equipment Kimwipes Kimberly-Clark 34155 All trans-Retinal (ATR) Sigma-Aldritch R2500 Polypropylene Drosophila vials Genesee Scientific 32–120 Droso-Plugs, narrow Genesee Scientific 59–200 Pyrex 9 well glass spot plates Fisher Scientific 13–748B Bel-Art SP Scienceware wide mouth wash bottles Fisher Scientific 22–288654 Bel-Art SP Scienceware Trigger Sprayers Fisher Scientific 01–189–100 Craft Smart round brush The Michaels Companies 10408282 Bell-Howell digital camcorders Bell-Howell DNV17HDZ Tripods for mounting cameras - - Cold plate TE Technology CP-031 Cold plate temperature controller TE Technology TC-48-20 Cold plate power supply TE Technology PS-12-8.4A Laminated aluminum shim (cut 7.5x11.5mm) Global Equipment WBB512969 Dino-Lite Edge digital microscopes Dino-Lite AM4115TW-GFBW Ingredients for Drosophila media NutriSoy soy flour Genesee Scientific 62–115 Yellow cornmeal Genesee Scientific 62–101 Drosophila agar type II Genesee Scientific 66–104 Inactive dry yeast Genesee Scientific 62–107 Dry Molasses Genesee Scientific 62–119 O-phosphoric acid Fisher Scientific A242–212 Propionic acid Fisher Scientific A258–500 Software ImageJ https://imagej.nih.gov/ij/ - Video to Video converter http://www.videotovideo.org/ - Transcriptomic Data Microarray gene expression profiling of isolated class III Drosophila dendritic arborization sensory neurons NCBI GEO GSE69353 Open in a separate window Materials & Reagents for running the described CURE.

Techniques: CRAfT Assay, Software, Microarray, Expressing, Isolation

Journal: Journal of Neuroinflammation

Article Title: Increased neuroinflammatory and arachidonic acid cascade markers, and reduced synaptic proteins, in brain of HIV-1 transgenic rats

doi: 10.1186/1742-2094-8-101

Figure Lengend Snippet: (A) Representative immunoblot of gp120 and tat protein in HIV-1 Tg rat brain (A), detected as described in Methods . mRNA levels of brain GFAP (B) and CD11b (D) in control and HIV-1 Tg rat brain, measured using real time RT-PCR, normalized to β-globulin and relative to control level (calibrator) using the ΔΔC T method. Representative immunoblots of (C) GFAP and (E) CD11b protein in control and HIV-1 Tg rat brain. Bar graphs are ratios of optical densities of individual protein bands to β-actin, expressed as percent of control. Data represent mean ± SEM, statistical significance: **p < 0.01, ***p < 0.001 as determined by unpaired t-test.

Article Snippet: Cytosolic protein blots were incubated overnight in Tris-buffered-saline containing 5% nonfat dried milk and 0.1% Tween-20, with specific primary antibodies for proinflammatory cytokines: IL-1β (1:500), TNFα (1:500); astrocytes: glial fibrillary acidic protein (GFAP) (1:1000); CD11b (1:1000); AA cascade proteins: cPLA 2 -IVA, sPLA 2 -IIA, iPLA 2 -VIA, COX-1 (1:1000), COX-2 (1:1000), cytochrome p450 epoxygenase, TXS, 5-, 12-, 15-LOX, mPGES, cPGES (1:1000); gp120 (1:100); tat (1:100); drebrin (1:1000), BDNF (1:1000) (Santa Cruz, Santa Cruz, CA);); neurofilament-L (1:500) (Cell Signaling Technology, Danvers, MA) and β-actin (1:10,000) (Sigma Aldrich, St. Louis, MO).

Techniques: Western Blot, Control, Quantitative RT-PCR